Abcam, ab60849, Recombinant human PKC eta protein

Size: 5µg / 10µg
Recombinant human PKC eta protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Active,
Accession:P24723,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

Product details:
ab204875
(PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein kinase C eta (PKC eta) is an enzyme also known as PKC η which belongs to the PKC family of serine/threonine kinases. This protein has a molecular mass of approximately 80 kDa and shows expression in numerous tissues with strong presence in epithelia and the immune system. PKC eta's mechanical function involves phosphorylation of serine and threonine residues on cellular target proteins modulating their activity. It plays a significant role in signal transduction pathways that govern various cellular processes.
Biological function summary
PKC eta involves itself in cellular differentiation and proliferation. It is a component of signal transduction complexes that mediate these processes. In epithelial cells PKC eta modulates cell adhesion and integrity. It also affects immune responses through its action in T-cells and other immune components impacting both innate and adaptive immunity. The enzyme interacts closely with other PKC isoforms allowing it to regulate diverse biological functions through complex networks.
Pathways
PKC eta plays an integral role in growth factor and cytokine signaling pathways. MAPK/ERK and PI3K/AKT pathways include PKC eta as key components for cell survival and proliferation. These pathways facilitate interactions and regulation involving proteins like RAF-1 and AKT coordinating cell growth and apoptosis. PKC eta's positioning in these pathways denotes its significance in maintaining cellular homeostasis and responding to external stimuli.
PKC eta implicates itself in autoimmune conditions like rheumatoid arthritis and in certain types of cancer such as breast cancer. PKC eta influences inflammatory responses often exacerbating autoimmune phenomena by interacting with cytokines and immune receptors. In cancer it may contribute to tumorigenesis by promoting cell survival through PI3K/AKT pathway engagement. It connects with proteins like NF-kB driving pathological changes in cell behavior and immune modulation.