Abcam, ab60867, Recombinant human HIPK1 protein

Size: 10µg
Recombinant human HIPK1 protein is a Human Fragment protein, in the 156 to 555 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Active,
Accession:Q86Z02,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

Product details:
ab64311
(Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIPK1 also known as Homeodomain-Interacting Protein Kinase 1 functions as a serine/threonine kinase. It has a molecular mass of approximately 132 kDa. This protein acts in the regulation of transcription factors and apoptosis. HIPK1 is widely expressed with higher levels in the brain heart and lung tissues. Researchers sometimes investigate its roles in several signal transduction pathways due to its regulatory functions.
Biological function summary
HIPK1 participates in various cellular processes including apoptosis DNA damage response and Wnt signaling. It forms complexes with several transcription factors such as p53 and Smad proteins modifying their activity to influence cell fate. By interacting with transcription regulators HIPK1 modulates gene expression and has implications in cell growth and differentiation.
Pathways
HIPK1 interacts with proteins in important signaling cascades including the Wnt and JNK pathways. Notably HIPK1 phosphorylates components like c-Jun influencing the JNK pathway's role in stress response and apoptosis. Additionally in the Wnt pathway HIPK1 modulates β-catenin signaling which is important for regulating cell proliferation and differentiation. These interactions connect HIPK1 to wider cellular communications and responses.
Researchers have linked HIPK1 to cancer and fibrotic diseases. Abnormal HIPK1 expression or activity can influence oncogenic processes due to its regulatory role on p53 a protein important for tumor suppression. Moreover in fibrosis altered HIPK1 activity impacts the TGF-β pathway by interacting with Smad proteins which promotes fibrogenesis. Understanding these connections could facilitate targeting HIPK1 in therapeutic strategies for these conditions.