Abcam, ab61, Anti-AP2 alpha + beta antibody [A6/2/2]

Size: 50µg
Mouse Monoclonal Transcription factor AP-2-alpha antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TFAP2A aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:A6/2/2,
Isotype:IgG1,
Light chain type:unknown,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TFAP2A aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P05549,
Specificity:Reacts with both AP-2 alpha and beta forms. As the antibody also recognises AP2 beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AP2 alpha + beta also known as adaptor protein complex 2 functions as part of the protein coat complex that facilitates clathrin-mediated endocytosis. Composed of alpha and beta subunits this complex has an approximate mass of 200 kDa. You can find AP2 alpha + beta highly expressed in cells with active endocytic processes such as neurons and epithelial cells.
Biological function summary
AP2 alpha + beta mediates the recruitment and assembly of clathrin-coated vesicles at the plasma membrane. This complex interacts directly with clathrin and cargo receptors playing an important role in cargo selection during endocytosis. It has an important role in cellular nutrient uptake and receptor internalization which are necessary for maintaining cellular function and signaling.
Pathways
Functions of AP2 alpha + beta are integral to the endocytic pathway and intracellular trafficking. It interacts with proteins such as clathrin and dynamin to ensure proper vesicle maturation and scission. You can find it involved in pathways that regulate cell surface receptor levels and synaptic vesicle recycling highlighting its interaction with proteins like AP180 and Eps15.
AP2 alpha + beta involvement correlates with neurological disorders and cancer. Abnormal function or expression of these proteins has been observed in Alzheimer’s disease where altered endocytosis affects amyloid precursor protein processing. Additionally AP2 alpha + beta mutations or dysregulation has connections to tumor progression often linked with oncogenic receptor endocytosis.