Abcam, ab6211, Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

Size: 50µL
Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody detecting Tyrosine Hydroxylase in Western Blot, IHC-Fr, ICC/IF . Suitable for Mouse, Rat . - Over 70 publications - Trusted since 2000
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-FoFr, ICC/IF, WB, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat TH aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P04177,
Specificity:Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.

Product details:
Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-FoFr, IHC-Fr, WB in mouse samples.
Anti-Tyrosine Hydroxylase antibody (ab6211) has been cited over 77 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Tyrosine Hydroxylase antibody (ab6211) has high sensitivity and specificity.
Anti-Tyrosine Hydroxylase antibody (ab6211) has 4 independent reviews from customers.
Anti-Tyrosine Hydroxylase antibody (ab6211) specifically detects Tyrosine Hydroxylase (UniProt ID: P04177; Molecular weight: 56kDa) and is sold in a 50 µL selling size.
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-pH: 7 - 7.4Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.
Biological function summary
Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.
Pathways
Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.
Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.