Abcam, ab6314, Anti-Cathepsin L + V antibody [33/2]

Size: 100µg
Mouse Monoclonal Cathepsin L/MEP antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 41 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:33/2,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).,
Specificity:Reacts specifically with the native and denatured forms of human cathepsin L (25kD) and procathepsin L (42 kD). Does not react with human cathepsin types B1, D1, H and S1, procathepsins B and D1 and recombinant procathepsin H, rat cathepsin B1, and mouse procathepsins B1 and D1.

Product details:
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cathepsin L also referred to as CTSL and Cathepsin V known as Cathepsin L2 or CTSV are lysosomal cysteine proteases that work in the proteolytic processing of proteins. Cathepsin L has an approximate mass of 37 kDa while Cathepsin V is slightly larger. Both enzymes localize mainly to lysosomes but they also occur in secretory vesicles and extracellular spaces under specific conditions. Expression of Cathepsin L spans various tissues including the liver spleen and kidney. Cathepsin V expression the highest in the thymus and testis and less so in other tissues.
Biological function summary
Cathepsin L and Cathepsin V play essential roles in protein degradation and turnover. They do not form stable complexes but can transiently interact with specific inhibitors to modulate their activity. These proteases maintain cellular homeostasis and assist in antigen processing facilitating immune responses. In particular Cathepsin V shows a more specialized role in extracellular matrix remodeling due to its broad substrate specificity and tissue distribution.
Pathways
Cathepsin L and Cathepsin V are active in the lysosomal degradation pathway. They collaborate with proteases such as Cathepsin B and Cathepsin S. Cathepsin L is also important for MHC class II-mediated antigen presentation affecting immune signaling. Due to its ability to process hormone precursors Cathepsin L impacts the insulin signaling pathway sharing functional relations with proteases like furin and prohormone convertases.
Cathepsin L associates with cancer progression and metastasis due to its role in matrix degradation and cell invasion. Overexpression or aberrant activity of Cathepsin L links to invasive cancer phenotypes. Cathepsin V implicates autoimmune diseases related to antigen processing defects particularly affecting thymic selection processes. Both proteases also have indirect connections to Alzheimer’s disease pathology due to their interactions with proteins such as ß-amyloid precursors.