Abcam, ab63828, Recombinant E. coli RuvC protein (Active)

Size: 20µg
Recombinant E. coli RuvC protein (Active) is a Escherichia coli K-12 Full Length protein, expressed in Escherichia coli, with >90%, suitable for ELISA, WB, FuncS, SDS-PAGE.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:SDS-PAGE, FuncS, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Active,
Accession:P0A814,
Animal free:No,
Carrier free:No,
Species:Escherichia coli K-12,
Storage buffer:pH: 6 - 8.5Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.158% Tris HCl, 0.0584% EDTA, 0.039% 2-Mercaptoethanol

Product details:
ab63828 can be used for: 1) Studies on the homologous recombination mechanism. 2) To use as an endonuclease which functions specifically to the Holliday structure.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RuvC also known as Holliday junction resolvase functions as an endonuclease that resolves Holliday junctions during DNA recombination and repair. It cleaves these four-way junctions to produce linear DNA duplexes promoting genetic diversity and genome stability. RuvC is a small protein with a mass of around 19 kDa. It is generally expressed in prokaryotes such as Escherichia coli and can localize to regions of DNA that undergo active recombination processes.
Biological function summary
RuvC ensures proper segregation of homologous chromosomes by processing DNA during cell division. It operates as a part of the RuvABC complex which consists of the RuvA and RuvB proteins in addition to RuvC. RuvA binds to the Holliday junctions and recruits RuvB forming a branch migration motor. RuvC then cleaves the junctions resolving them. This activity plays a major role in maintaining genetic stability by enabling the accurate exchange of genetic material.
Pathways
RuvC contributes significantly to homologous recombination and DNA repair mechanisms. It participates within the homologous recombination repair pathway intertwining with proteins such as RecA which facilitates the alignment of homologous DNA sequences and RecBCD a helicase/nuclease involved in processing DNA double-strand breaks. RuvC acts after RecA-mediated strand invasion to resolve recombination intermediates.
Impaired function of proteins homologous to RuvC in eukaryotes can relate to genomic instability and cancer. For example the absence or dysfunction of similar pathways in humans can lead to hereditary nonpolyposis colorectal cancer (HNPCC) where proteins like MLH1 part of the homologous recombination repair machinery are involved. These linkages highlight the critical nature of Holliday junction resolution in preventing unrepaired DNA damage accumulation reducing the risk of oncogenic transformations.