Abcam, ab63996, Recombinant HIV1 Nef protein

Size: 20µg
Recombinant HIV1 Nef protein is a Human immunodeficiency virus type 1 (BRU ISOLATE) Full Length protein, in the 1 to 206 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P03406,
Animal free:No,
Carrier free:No,
Species:Human immunodeficiency virus type 1 (BRU ISOLATE),
Storage buffer:pH: 6 - 8.5Constituents: 50% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.29% Sodium chloride, 0.078% 2-Mercaptoethanol

Product details:
ab63996 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIV1 Nef also known as Nef HIV Nef protein and Nef HIV protein is a small myristoylated protein with a mass of approximately 27 kDa. It expresses predominantly in the cytoplasm of HIV-1 infected cells. HIV1 Nef plays an important role in viral pathogenicity by manipulating host cell machinery to favor viral replication and immune escape. The protein affects the host cell's plasma membrane and endosomal compartments where it modifies the trafficking of various cell surface molecules.
Biological function summary
Nef affects several cellular processes to promote viral persistence and disease progression. It disrupts normal cellular signaling and acts as part of larger protein complexes that interact with host proteins. For instance it is known for its interaction with proteins like AP-1 which Nef uses to alter the re-routing of MHC-I molecules to lysosomes. This results in reduced exposure of infected cells to the immune system and highlights its role in immune evasion.
Pathways
Nef engages in interference with immune and cellular signaling pathways. It tightly interacts with the PI3K/Akt signaling pathway important for cell survival and proliferation thereby assisting the virus in sustaining a hospitable cellular environment. Nef also partners with Src family kinases further supporting its functions in reprogramming cellular processes and enhancing viral replication.
HIV1 Nef is intimately related to HIV/AIDS pathogenesis. It contributes significantly to the immunodeficiency observed in infected individuals by modulating CD4 and CTLA-4 downmodulation affecting immune signaling and response. Furthermore Nef aligns with Tat protein in HIV-infected cells enhancing the pathological effects seen in the neurodysfunction often associated with disease progression illustrating its role in complex interactions that worsen HIV-related complications.