Abcam, ab64085, Anti-TMEM16A antibody [SP31]

Size: 100µL / 500µL / 1mL
Anti-TMEM16A antibody [SP31] (ab64085) is a rabbit monoclonal antibody detecting TMEM16A in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications - Trusted since 2008
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP31,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-TMEM16A antibody [SP31] (ab64085) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of TMEM16A?
Anti-TMEM16A [SP31] (ab64085) specifically detects a band for TMEM16A (UniProt: Q5XXA6) at a molecular weight of 114kDa.
Collaboration
Anti-TMEM16A [SP31] (ab64085) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Trusted by the scientific community
Anti-TMEM16A [SP31] (ab64085) was first used in a scientific publication in 2008 and has been cited over 50 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [SP31] also available for your convenience: ab64085, Carrier free -
ab198412
, Alexa Fluor® 647 -
ab267509
, PE -
ab267510
, APC -
ab319337
, Alexa Fluor® 488 -
ab319587
, Alexa Fluor® 594 -
ab319830
, Alexa Fluor® 555 -
ab319971
, Alexa Fluor® 750 -
ab321687
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM16A also known as ANO1 is a transmembrane protein functioning as a calcium-activated chloride channel. It plays a significant role in the regulation of membrane potential and fluid secretion. TMEM16A has a molecular mass of approximately 114 kDa. This protein is expressed in various tissues including airway epithelial cells intestinal epithelium and smooth muscle cells. Its expression is also noticeable in parts of the nervous system indicating its widespread physiological importance.
Biological function summary
TMEM16A contributes to processes such as epithelial fluid transport and smooth muscle contraction. It is an integral component of a calcium-sensitive chloride conductance. This channel regulates ion transport across epithelial cell membranes influencing fluid secretion in airways and gastrointestinal tracts. In blood vessels TMEM16A helps control vascular tone by mediating smooth muscle cell contraction which is dependent on intracellular calcium levels.
Pathways
TMEM16A interacts with key cellular signaling cascades especially the calcium signaling pathway. This interaction has critical implications for chloride conductance in epithelial cells and smooth muscle function. TMEM16A is linked with proteins like calcium/calmodulin-dependent protein kinase II (CaMKII) which further modulates its activity. Additionally TMEM16A is implicated in pathways regulating mucin secretion which involves other proteins like cystic fibrosis transmembrane conductance regulator (CFTR).
TMEM16A plays a role in respiratory diseases like asthma and cystic fibrosis where dysregulation in chloride channels leads to abnormal mucus secretion and airway obstruction. It also relates to hypertension due to its role in vascular tone modulation. Research shows TMEM16A interacts with proteins like CFTR in cystic fibrosis affecting chloride transport and fluid balance. In hypertension its interaction with calcium-sensing proteins further complicates vascular responsiveness and pressure regulation.