Abcam, ab64088, Anti-CD20 antibody [SP32]

Size: 20µL / 100µL / 500µL / 1mL
Anti-CD20 antibody [SP32] (ab64088) is a rabbit monoclonal antibody detecting CD20 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, mIHC . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP32,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Dog, Human, Cat, Sheep,
Applications:IP, ICC/IF, Flow Cyt (Intra), mIHC, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD20 antibody [SP32] (ab64088) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB, mIHC in human, mouse, rat samples.
Anti-CD20 antibody [SP32] (ab64088) has been cited over 24 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD20 antibody [SP32] (ab64088) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-CD20 antibody [SP32] (ab64088) has been confirmed by testing in knockout samples.
Anti-CD20 antibody [SP32] (ab64088) has 12 independent reviews from customers.
Anti-CD20 antibody [SP32] (ab64088) specifically detects CD20 (UniProt ID: P11836; Molecular weight: 33kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL, 500 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone SP32 -
ab236434
Antibody clone SP32 is also available pre-conjugated to a variety of labels for your convenience - PE, APC, HRP, Alexa Fluor® 488, Alkaline Phosphatase, Alexa Fluor® 594, Alexa Fluor® 555, Alexa Fluor® 568 (
ab305647
ab305648
ab305649
ab307132
ab308806
ab310536
ab312067
ab312544
Anti-CD20 antibody [SP32] (ab64088) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from TCS by protein A/G., Storage buffer-pH: 7.6Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.