Abcam, ab64672, APC Anti-Transferrin Receptor antibody [MEM-75]

Size: 25Test / 50Test
Mouse Monoclonal Transferrin Receptor antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing TFRC protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:MEM-75,
Isotype:IgG1,
Light chain type:kappa,
Conjugation:APC,
Excitation/Emission:Ex: 650nm, Em: 660nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing TFRC protein. The exact immunogen used to generate this antibody is proprietary information.P02786,
Specificity:ab64672 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. ab64672 does not block binding of transferrin to the receptor.

Properties and Storage Information:
Form-Liquid, Purification technique-Size-exclusion chromatography, Purification notes-The purified antibody (>95% by SDS-PAGE) is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use., Storage buffer-pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.
Biological function summary
TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.
Pathways
The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.
Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.