Abcam, ab64960, Anti-p35 antibody

Size: 100µg
Rabbit Polyclonal CD5R1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CDK5R1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CDK5R1. The exact immunogen used to generate this antibody is proprietary information.Q15078

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p35 protein also known as p35 form is a regulatory subunit of the cyclin-dependent kinase 5 (Cdk5) complex. It weighs approximately 35 kDa. Expressed predominantly in the central nervous system p35 plays an important role in neural development and synaptic function. It binds to and activates Cdk5 an enzyme involved in various processes critical to neuronal function.
Biological function summary
P35 is essential for the regulation of many aspects of neural development including cell migration and axonal growth. It forms a complex with Cdk5 which then phosphorylates various substrates that are critical for these processes. The p35/Cdk5 complex influences the cytoskeletal dynamics needed for neuronal positioning and dendritic spine formation and it is significant for learning and memory.
Pathways
P35 serves in the neuronal signaling and cell cycle pathways. The Cdk5/p35 complex interfaces with pathways such as those involving the neurofilament proteins important for maintaining neuronal structure and function. In the cell cycle though atypical Cdk5/p35 can affect pathways related to cell cycle arrest highlighting its diverse functional connectivity.
P35 is significantly involved in neurodegenerative diseases like Alzheimer's and amyotrophic lateral sclerosis (ALS). In Alzheimer’s abnormal p35 cleavage to p25 by enzymes like calpain results in hyperactivity of Cdk5 leading to neuronal damage. The similarity in pathways also connects p35 to other neuronal proteins such as tau where misregulation can result in tauopathy a feature observed in several neurodegenerative disorders.