Abcam, ab6514, Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

Size: 100µg
Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) is a mouse monoclonal antibody detecting HSV 1 ICP4 Immediate Early Protein in Western Blot, ICC/IF, ELISA . Suitable for Herpes simplex virus . - Over 60 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:10F1,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Herpes simplex virus,
Applications:ICC/IF, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:ELISA titer 1/25,600 absorption of 0.10

Product details:
What is this antibody validated in?
Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Herpes simplex virus samples.
Trusted by the scientific community
Anti-HSV 1 ICP4 Immediate Early Protein [10F1] (ab6514) was first used in a scientific publication in 2002 and has been cited over 60 times in peer-reviewed journals.
Reviewed by scientists
Anti-HSV 1 ICP4 Immediate Early Protein [10F1] (ab6514) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HSV 1 ICP4 Immediate Early Protein also known as Infected Cell Protein 4 (ICP4) has a significant mechanical role during the herpes simplex virus type 1 (HSV-1) infection. ICP4 has a molecular mass of approximately 175 kDa. It uniquely expresses in the early phase of HSV-1 infection in the host cells primarily in the nucleus where it functions as a transcriptional activator. Its expression tightly regulates the viral replication cycle making it an essential protein for the virus.
Biological function summary
HSV-1 ICP4 plays a critical role as a major regulator of viral gene expression. ICP4 acts within a transcriptional complex where it binds to specific DNA sequences to activate the transcription of early and late viral genes. The protein can directly interact with host transcription machinery modifying the host cell's transcriptional dynamics. This ability allows the virus to hijack cellular machinery efficiently advancing its replication and further propagation within the host.
Pathways
HSV-1 ICP4 integrates into key transcriptional pathways that govern viral gene expression. The protein is pivotal in regulating both the early and late gene expression pathways of HSV-1 establishing a coordinated effort for viral replication. ICP4 associates with other viral proteins like ICP0 and ICP27 which function collaboratively within these pathways to modulate the synthesis of viral DNA and proteins necessary for successful virus assembly and release.
HSV-1 ICP4 strongly links to conditions such as herpes simplex virus infection and associated complications including encephalitis. During infection ICP4 contributes to the pathogenesis by promoting viral replication and persistence in host cells. Its interaction with the protein ICP27 further associates with the inhibition of host cell apoptosis sustaining viral survival and enhancing infection severity. These connections highlight its relevance in antiviral research and therapy development where targeting ICP4 could mitigate HSV-1-related diseases.