Abcam, ab65329, Total Antioxidant Capacity Assay Kit

Size: 100Test
Total Antioxidant Capacity Assay Kit ab65329 can measure either the combination of both small molecule antioxidants and proteins, or small molecules alone in the presence of our proprietary Protein Mask. Simply add Protein Mask reagent if required, then incubate with Cu2+ solution for 90-mins at room temperature. Readout on any colorimetric (570 nm) plate reader. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Assay time:2h,
Assay Platform:Microplate reader

Product details:
Total Antioxidant Capacity Assay Kit ab65329 can measure either the combination of both small molecule antioxidants and proteins, or small molecules alone in the presence of our proprietary Protein Mask.
In the total antioxidant capacity assay protocol, the Cu2
ion is converted to Cu
by both small molecule and protein antioxidants. The Protein Mask prevents Cu2
reduction by proteins, enabling the analysis of only the small molecule antioxidants. The reduced Cu
ion is chelated with a colorimetric probe giving a broad absorbance peak around 570 nm, proportional to the total antioxidant capacity.
Total antioxidant capacity assay protocol summary:
- add protein mask to samples if only measuring small molecule total antioxidant capacity
- add samples and standards to wells
- add Cu2
solution and incubate for 90 min at room temp
- analyze with microplate reader
Antioxidants play an important role in preventing the formation of, and scavenging of, free radicals and other oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). Trolox is used to standardize antioxidants, with all other antioxidants being measured in Trolox equivalents. Measurement of the combined non-enzymatic antioxidant capacity of biological fluids and other samples provides an indication of the overall capability to counteract reactive oxygen species (ROS), resist oxidative damage and combat oxidative stress-related diseases.
Related antioxidant capacity assays and oxidative stress products
The most popular antioxidant capacity assay is Total Antioxidant Capacity Assay Kit ab65329, based on copper reduction. We also supply FRAP Assay Kit
ab234626
based on iron reduction, DPPH Antioxidant Assay Kit
ab289847
based on reduction of the DPPH probe, and ORAC Assay Kit
ab233473
Read about our full range of
oxidative stress assays here
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Please refer to protocols