Abcam, ab65339, Creatine Assay Kit (Colorimetric/Fluorometric)

Size: 100Test
Creatine Assay Kit ab65339 is a quantitative, mix-and-read assay with one 60-min incubation step. In the assay, creatine is acted on by creatinase, followed by the action of sarcosine oxidase and HRP. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 538/587 nm) plate reader. - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Sensitivity:> 0.001 mM,
Range:0.001 - 10 mM,
Assay time:1h,
Assay Platform:Microplate reader

Product details:
Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339) provides an accurate, convenient measure of creatine in a variety of biological samples.
Creatine assay principle
In the creatine assay, creatine is enzymatically converted to sarcosine by creatinase. Sarcosine is then oxidized by sarcosine oxidase producing hydrogen peroxide. Hydrogen peroxide is then processed by a peroxidase causing a reaction with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). Detection is by either colorimetric or fluorometric plate reader.
This is the same method used in the creatinine assay kit
ab65340
, except that this kit ab65339 does not include the initial creatininase enzyme step.
Creatine assay protocol summary
- add reaction mix to sample and standard wells
- incubate for 60 min
- analyze with a microplate reader
Related and recommended products
Related assay kits for the study of creatine and creatinine biology include:
- Creatinine assay kit
ab65340
- Creatine kinase activity assay kit
ab155901
See the product datasheet for
ab65340
for a description of our assays for measuring the albumin-creatinine ratio and urea creatinine ratio.
Other notes
This product was previously called K635 Biovision Creatine Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C