Abcam, ab65866, Human IgE protein (Azide free)

Size: 100µg / 1mg
Human IgE protein (Azide free) is a Human Full Length IgE protein with >90% purity, < 25 EU/mg endotoxin level and suitable for western blot, ELISA, IHC, ICC and SDS-PAGE. The predicted molecular weight of ab65866 protein is 185 kDa. - Save time and ensure accurate results - use ab65866 as an IgE isotype control - Optimal bioactivity, stability and reproducibility - No contamination from other immunoglobulin isotypes - Available in different sizes to fit your experimental needs
Key facts
Purity:>90% SDS-PAGE,
Endotoxin level:< 25 EU/mg,
Expression system:Native,
Tags:Tag free,
Applications:ICC, IHC - Wmt, ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:1 mg of ab65866 is 380,000± 50,000 IU/ml.,
Accession:P0DOX4,
Animal free:No,
Carrier free:Yes,
Species:Human,
Storage buffer:pH: 7.4Constituents: PBS, 0.88% Sodium chloride

Product details:
Ensure the validity of your result using our Immunoglobulin E (IgE) protein ab65866 as a control.
IgE protein ab65866 can be used as a positive control in SDS-PAGE and western blot assays.
Analyze your ELISA data using the IgE ab65866 protein to generate and plot the standard curve.
Check out our ELISA protocol for more information
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:
- Low batch-to-batch variation
- Higher degree of purity (>90%)
- A more uniform product (monoclonal and not polyclonal)
Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.
Ensure the validity of your result using our Immunoglobulin E (IgE) protein ab65866 as an isotype control. ab65866 can be used as a positive control in SDS-PAGE and western blot assays.
Analyze your ELISA data using the IgE ab65866 protein to generate and plot the standard curve.
Check out our ELISA protocol for more information here https://www.abcam.com/en-us/technical-resources/protocols/indirect-and-direct-elisa.
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line.
Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients.
A comparison which is highly superior on the following key parameters:
- Low batch-to-batch variation
- Higher degree of purity (>90%)
- A more uniform product (monoclonal and not polyclonal)
Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The IgE protein [also known as immunoglobulin E] is a type of antibody with a molecular mass of approximately 190 kDa. IgE is expressed primarily on the surface of mast cells and basophils. It plays a central role in the immune system by binding to allergens and triggering the release of inflammatory mediators from mast cells and basophils. IgE's high-affinity binding to its receptor FcεRI on these effector cells facilitates this process. IgE's activity is central to initiating immediate hypersensitivity reactions which are a part of the body's immune response.
Biological function summary
The IgE protein functions as a mediator in allergic reactions. It does not operate as part of a complex but directly interacts with allergens and the IgE-specific receptors on immune cells. IgE's role is important in defense against parasitic infections where it performs a protective function by promoting eosinophil activation and degranulation. This action helps the body to expel the parasites. In the absence of parasitic infection IgE's function translates into its role in allergic responses where it becomes responsible for the symptoms associated with allergic diseases.
Pathways
IgE is an important player in the immune response pathway. The protein interacts with allergy-related pathways such as the T helper 2 (Th2) pathway. IgE's role in this pathway involves cooperation with cytokines notably IL-4 and IL-13 which are produced by Th2 cells and promote IgE synthesis in B cells. Also IgE is linked to the FcεRI receptor signaling pathway that initiates the release of histamines and other mediators when IgE binds to an allergen leading to an allergic response.
IgE is closely linked to allergic conditions like asthma and allergic rhinitis. These conditions result from IgE-mediated inflammatory responses that affect the respiratory system. In asthma IgE antibodies play a role in airway inflammation and hyperreactivity by promoting the activation of multiple immune cells including eosinophils and mast cells. IgE's involvement in allergic rhinitis manifests through similar mechanisms with nasal inflammation and irritation. IgE's interaction with other proteins such as histamine-releasing factor exacerbates these conditions by increasing the release of histamine which intensifies the allergic symptoms.