Abcam, ab66031, Anti-Twist2 antibody

Size: 100µg
Rabbit Polyclonal Twist2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Recombinant full length protein - Human samples. Cited in 28 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is able to detect both Twist1 and Twist2 proteins. We have observed weak detection of the Twist1 protein using Twist1 recombinant proteins along with select lysates generating a BOI of weak signal associated to Twist1. The signal detected in the endogenous lysates and recombinant proteins is much greater for Twist2.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Twist2 also known as Dermo-1 is a basic helix-loop-helix (bHLH) transcription factor with a molecular mass of approximately 18 kDa. It is expressed in various tissues including mesodermal and neural crest-derived tissues such as the heart craniofacial structures and limb buds. Twist2 has a mechanical role in transcription regulation where it binds to E-box sequences within DNA modulating gene expression involved in cell lineage differentiation and development.
Biological function summary
Twist2 functions by influencing cellular processes like differentiation proliferation and apoptosis. It often acts as a negative regulator of myogenesis and osteogenesis. Twist2 is part of a larger protein complex that includes different partners like E-proteins allowing for combinatorial control over target gene expression. Its activity often results in the promotion of epithelial-to-mesenchymal transition (EMT) an important process in both normal development and pathological conditions.
Pathways
Twist2 plays a significant role in the TGF-β signaling pathway and the Wnt signaling pathway. These pathways are important in regulating embryonic development and maintaining adult tissue homeostasis. Twist2's interaction with proteins such as Smads in the TGF-β pathway and β-catenin in the Wnt pathway highlights its involvement in mediating complex signaling networks necessary for mesenchymal cell transition and maintenance.
Twist2's dysregulation is linked to conditions such as Saethre-Chotzen syndrome and certain types of cancer including breast cancer. Its connection to the Snail family of transcription factors relates Twist2 to cancer progression through the promotion of invasiveness and metastasis. Additionally Twist2 mutations can be associated with craniosynostosis syndromes where it affects the regulation of bone development in conjunction with other factors like FGFR.