Abcam, ab6640, Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

Size: 100µg
Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) is a rat monoclonal antibody detecting F4/80 in Flow Cytometry, ICC/IF . Suitable for Mouse . - Over 1090 publications - Trusted since 2002
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:CI:A3-1,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing Adgre1 protein. The exact immunogen used to generate this antibody is proprietary information.Q61549,
Specificity:Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.

Product details:
Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra) and ICC/IF.
Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) was first used in a scientific publication in 2003 and has been cited over 1093 times in peer reviewed journals. It's performance in IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) has high sensitivity and specificity.
Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) has 68 independent reviews from customers.
Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) specifically detects F4/80 (UniProt ID: Q14246; Molecular weight: 96kDa) and is sold in 100 µg selling sizes.
Antibody clone CI:A3-1 is also available pre-conjugated to a variety of labels for your convenience - APC, FITC, PE (ab158,
ab15155
ab15156
F4/80 is a key marker for macrophages, playing a significant role in immune response modulation and inflammation and is linked to various inflammatory diseases. Clone CI:A3 is top cited in the market and a well known immunophenotyping marker in mouse macrophages.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant., Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
F4/80 also known as fibroblast-specific protein (FSP1) and by its monoclonal antibody designation BM8 is an established murine macrophage marker. It is a glycoprotein with a molecular weight of approximately 160 kDa. F4/80 is predominantly expressed on the surface of tissue-resident macrophages in mice where it serves as an essential component of the immune system. These macrophages are present across various organs and tissues such as liver (Kupffer cells) lung (alveolar macrophages) spleen and the peritoneal cavity where its expression can be detected using techniques like f4/80 immunofluorescence and f4/80 staining.
Biological function summary
The protein functions in the immune response by promoting phagocytosis and the clearance of apoptotic cells. It contributes to mediating anti-inflammatory responses and is associated with the regulation of cellular immune responses. Although not part of a larger protein complex F4/80 plays a critical role in the differentiation and maturation of macrophages. It is often used as a reliable f4/80 marker in immunohistochemistry (IHC) to identify and study macrophages within tissues.
Pathways
The function of F4/80 influences the Toll-like receptor (TLR) signaling pathway and cytokine production regulatory pathways. These pathways contribute to the innate immune response by recognizing microbial pathogens and activating inflammatory responses. F4/80 interacts with other proteins such as CD11b which is also involved in these immune responses aligning their functions to regulate the activity of macrophages and coordinate with other immune cells.
F4/80's involvement in inflammation extends to its role in conditions like atherosclerosis and rheumatoid arthritis. In atherosclerosis the interaction between F4/80 and other proteins like CD36 leads to macrophage accumulation and plaque formation in blood vessels. In rheumatoid arthritis F4/80 expression on specific macrophages contributes to joint inflammation by mediating the secretion of pro-inflammatory cytokines. Both of these conditions highlight the significance of F4/80 in the pathogenesis and progression of diseases characterized by immune dysregulation.