Abcam, ab70259, Anti-Xrn1 antibody

Size: 50µL
Rabbit Polyclonal XRN1 antibody. Suitable for IP, WB, ICC/IF, EMSA and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human XRN1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, EMSA, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human XRN1. The exact immunogen used to generate this antibody is proprietary information.Q8IZH2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Xrn1 also known as 5'-3' Exoribonuclease 1 plays an important mechanical role in degrading RNA molecules. It is an enzyme with a molecular mass of approximately 212 kDa. Xrn1 is expressed in a wide range of eukaryotic organisms where it functions in the cytoplasm. It processes RNA molecules by degrading them from the 5' to the 3' end effectively participating in RNA turnover and quality control mechanisms. This activity highlights Xrn1's importance in cellular RNA regulation.
Biological function summary
The role of Xrn1 is fundamental in maintaining RNA stability and synthesis within eukaryotic cells. Xrn1 does not typically form part of a larger protein complex but rather operates independently in the cytoplasm. Its enzymatic function assists in removing defective redundant or obsolete RNAs ensuring that cells allocate resources only to functional and necessary RNA transcripts. This degradation process directly impacts mRNA turnover securing proper gene expression regulation.
Pathways
The enzymatic action of Xrn1 fits into the mRNA decay pathways notably the nonsense-mediated mRNA decay (NMD) and major mRNA degradation pathway. During NMD Xrn1 works alongside other proteins such as Upf1 which identifies faulty mRNAs for degradation. These pathways ensure that the genetic information is accurately translated into proteins by removing erroneous or incomplete transcripts. Xrn1's activity aligns with its critical involvement in maintaining cellular homeostasis through these pathways.
Alterations in Xrn1 function relate to conditions like cancer and neurological disorders. Misregulation of Xrn1 activity can lead to abnormal RNA accumulation contributing to uncontrolled cell proliferation in cancer. Additionally connections exist between Xrn1 and the protein TDP-43 particularly in neurodegenerative disorders where improper RNA processing can result in toxic RNA species accumulation. Studying Xrn1 function and its interactive partners could advance understanding and potential treatment approaches for these conditions.