Abcam, ab70301, Anti-p400 antibody

Size: 100µL
Rabbit Polyclonal p400 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human EP400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human EP400. The exact immunogen used to generate this antibody is proprietary information.Q96L91

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70301 was affinity purified using an epitope specific to p400 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p400 protein also known as EP400 plays a mechanical role as an ATPase in chromatin remodeling. Its molecular mass is approximately 420 kDa. This protein is predominantly expressed in tissues with high proliferative capacities such as testis and lymph nodes. It functions within the TIP60 complex also consisting of other important histone acetyltransferase components that regulate transcriptional activation and DNA repair processes.
Biological function summary
The p400 protein contributes significantly to gene expression and chromatin structure. It participates in the modification of chromatin architecture facilitating the access of transcription factors to DNA. p400 becomes an integral component of the TIP60 complex vital for the acetylation of nucleosomal histones which promotes transcriptional activation. Through these mechanisms p400 influences cellular processes such as cell cycle regulation and apoptosis.
Pathways
P400's functionality integrates into significant cellular pathways like the cell cycle and DNA repair pathways. Within the cell cycle p400's role in chromatin remodeling links it to the regulation of genes controlling cell proliferation. The protein interacts with histones and is part of complexes that include proteins such as TRRAP contributing to the DNA damage response and repair mechanisms. These connections highlight p400's involvement in cellular defense and maintenance of genomic stability.
P400's dysfunction associates with cancer and neurodegenerative disorders. Abnormal regulation of p400 due to its function in transcription and repair implicates its involvement in tumorigenesis particularly in cancers such as colorectal cancer. In neurological contexts aberrant p400 activity relates to neurodegenerative diseases like Alzheimer's where chromatin structure and gene expression go awry. The target’s interconnectedness with other proteins like TIP60 during the onset of these conditions highlights its importance in pathophysiological processes.