Abcam, ab70349, Anti-hCAP-D3 antibody

Size: 100µL
Rabbit Polyclonal hCAP-D3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NCAPD3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NCAPD3. The exact immunogen used to generate this antibody is proprietary information.P42695,
Specificity:We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HCAP-D3 also known as chromosome-associated protein D3 or CAP-D3 plays a role in the regulation of chromatid structure during cell division. With a molecular mass of approximately 160 kDa this protein is a component of the condensin II complex. hCAP-D3 is expressed in various tissues throughout the body prominently in cells that are dividing. Its expression changes can influence chromosomal segregation which is essential for proper cell cycle progression.
Biological function summary
HCAP-D3 influences the stabilization of chromosome architecture. It is a part of the condensin II complex working with other proteins like hCAP-H2 and hCAP-G2 to facilitate mitotic chromatid condensation. The correct function of hCAP-D3 during mitosis ensures proper separation of sister chromatids maintaining genomic integrity. The protein's mechanic role is important in preventing aneuploidy which can lead to various cellular malfunctions.
Pathways
HCAP-D3 participates in the mitotic cell cycle pathway significantly affecting chromosomal condensation processes. Its interaction with proteins like SMC2 and SMC4 is critical for the optimal function of the condensin II complex. Additionally hCAP-D3 engages in pathways associated with DNA repair and replication highlighting its importance in cell division and genomic stability. These interactions demonstrate its integration within cellular processes that preserve cell health.
HCAP-D3 associates with certain cancers due to its role in genomic stability. Aberrations in hCAP-D3 expression or function can lead to improper chromosomal segregation contributing to oncogenesis. Specifically research links hCAP-D3 to colorectal cancer where its altered activity may drive tumor progression. The protein also has connections to proteins involved in the control of mitotic checkpoints such as Aurora B highlighting its relevance in maintaining cell cycle fidelity and preventing disease.