Abcam, ab70374, Anti-RICTOR antibody

Size: 100µL
Rabbit Polyclonal RICTOR antibody. Suitable for IHC-P, IP, Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human RICTOR.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-P, IP, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RICTOR. The exact immunogen used to generate this antibody is proprietary information.Q6R327,
Specificity:Initial tests for reactivity with mouse RICTOR from NIH3T3 cells suggest that higher amounts of lysate and less dilution of ab70374 are required for brief exposure times.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RICTOR (Rapamycin-insensitive companion of mTOR) is an important component of the mTOR complex 2 (mTORC2) distinguished by its ability to interact with mTOR (mechanistic target of rapamycin). RICTOR possesses a molecular mass of approximately 192 kDa. You can find RICTOR widely expressed in various tissues including the brain liver and skeletal muscle. Researchers also refer to it as AVO3.
Biological function summary
The protein plays a significant role in cellular growth proliferation and survival. RICTOR forms part of the mTORC2 complex which is important for Akt/PKB phosphorylation. Activation of mTORC2 by RICTOR regulates actin cytoskeleton dynamics and membrane trafficking. This involvement affects the control of cell metabolism and immune responses.
Pathways
RICTOR integrates into the PI3K/Akt signaling pathway influencing cell growth and survival in response to nutrients and growth factors. RICTOR as part of mTORC2 also interacts with proteins such as Sin1 and mLST8 helping regulate PKB/Akt phosphorylation at Ser473. This interaction links RICTOR's function to the insulin signaling pathway which controls aspects of glucose metabolism.
The dysregulation of RICTOR plays a role in cancer progression and metabolic disorders. Alterations in the PI3K/Akt/mTOR signaling pathway can lead to the development of various cancers with RICTOR contributing to tumorigenesis through Akt activation. Additionally aberrant RICTOR expression affects insulin signaling therefore connecting it to diabetes. Through these pathways and conditions RICTOR's interaction with proteins such as mTOR illustrates its relevance in disease mechanisms and therapeutic targets.