Abcam, ab70454, Anti-DHX15/prp43 antibody

Size: 100µL
Rabbit Polyclonal DHX15/prp43 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DHX15.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DHX15. The exact immunogen used to generate this antibody is proprietary information.O43143

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70454 was affinity purified using an epitope specific to DHX15/prp43 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DHX15 also known as DEAH-Box Protein 15 or prp43 forms part of the DExH/D-box helicase family. This protein functions in the ATP-dependent unwinding of RNA and has a molecular mass of approximately 95 kDa. Expression of DHX15 is found in various tissues with high levels in heart and skeletal muscle. The protein is involved in the splicing of pre-mRNA and rRNA processing where its enzymatic activities ensure the proper modification and maturation of RNA molecules.
Biological function summary
The splicing complex involves DHX15 as an important component. This protein plays a role in the disassembly of spliceosomes leading to spliceosome recycling essential for efficient mRNA processing. DHX15 associates with other proteins within the spliceosome helping to coordinate the removal of introns from pre-mRNA. As part of the broader RNA processing machinery DHX15 ensures the accurate and efficient production of mature RNA necessary for proper gene expression and cellular function.
Pathways
DHX15 participates in the mRNA splicing and ribosome biogenesis pathways. It works alongside related proteins like SF3B1 within the spliceosome for mRNA splicing. Ribosome biogenesis involves DHX15 acting in concert with several factors to facilitate rRNA maturation impacting protein synthesis capacities of cells. These pathways which require precise RNA processing and protein assembly highlight DHX15's roles in cellular metabolism and regulatory mechanisms.
DHX15 associates with myelodysplastic syndrome and congenital heart defects. Mutations or dysregulation of DHX15 disrupt RNA processing contributing to these conditions. The protein SF3B1 interacts with DHX15 in myelodysplastic syndrome. In congenital heart defects disrupted DHX15 function correlates with improper heart and vascular formation during development. Understanding these associations points to the significance of DHX15 in maintaining RNA processing fidelity and its broader implications in human health.