Abcam, ab70722, Anti-SEC16A/SEC16 antibody

Size: 100µL
Rabbit Polyclonal SEC16A/SEC16 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SEC16A.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SEC16A. The exact immunogen used to generate this antibody is proprietary information.O15027

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SEC16A also known as SEC16 is an important component in the secretory pathway specifically in vesicle trafficking from the endoplasmic reticulum (ER) to the Golgi apparatus. This protein plays a significant role in the assembly of coat protein complex II (COPII)-coated vesicles acting as a scaffold for other components. SEC16A has a molecular mass of approximately 240 kDa. It is broadly expressed in various tissues with notable expression in cells with high secretory activity.
Biological function summary
Functions of SEC16A are critical in maintaining ER exit sites (ERES) where the budding of COPII vesicles occurs. It localizes to these ERES facilitating the formation of transport vesicles. SEC16A does not work alone; it interacts with several other proteins to form a functional multi-protein complex associated with this vesicle trafficking process. These interactions ensure the efficient transport of proteins and lipids necessary for cellular homeostasis and secretion.
Pathways
SEC16A integrates into the COPII-mediated vesicle formation pathway pivotal for protein sorting and transportation. This protein collaborates closely with the SAR1 SEC23 SEC24 SEC13 and SEC31 proteins through the vesicle formation cycle. Besides its role in COPII assembly SEC16A also contributes to the regulation of ER-Golgi transport influencing the growth factor signaling pathways that depend on the accurate delivery of their receptor components to the cell surface.
Disruptions in the function of SEC16A links to conditions like Anderson-Fabry disease and congenital disorders of glycosylation. Abnormal vesicle trafficking due to SEC16A impairment can lead to an accumulation of metabolites or misfolded proteins that disrupt cellular function. SEC16A's relationship with proteins involved in lipid metabolism and glycosylation supports the role in these disorders. Understanding the behaviour of SEC16A in these contexts may contribute to targeted therapeutic strategies.