Abcam, ab70781, Anti-PRP16 antibody

Size: 100µL
Rabbit Polyclonal PRP16 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DHX38 aa 1200 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DHX38 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q92620

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70781 was affinity purified using an epitope specific to PRP16 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRP16 also known as RNA-dependent ATPase PRP16 is an essential component in pre-mRNA splicing. It functions mechanically as an ATP-dependent RNA helicase facilitating conformational changes in the spliceosome. This protein has a molecular mass of approximately 120 kDa. PRP16 expression occurs largely in the nucleus where it participates in RNA processing events.
Biological function summary
PRP16 plays a critical role in the rearrangement of spliceosomal components during the second catalytic step of splicing. It is a part of the spliceosome complex and is necessary for the accurate removal of introns from pre-mRNA. This protein ensures the proper transition of the spliceosome from the C complex to the B* complex therefore influencing mRNA maturation and stability.
Pathways
PRP16 operates within the mRNA processing and splicing pathways. It is closely related to other spliceosomal proteins such as PRP8 and SF3B1 which coordinate to ensure efficient gene expression regulation through precise splicing events. These interconnected pathways highlight PRP16’s indispensable function in gene expression and RNA management.
PRP16 exhibits a connection with genetic conditions involving splicing defects. Mutations or dysregulation of this helicase may relate to cancers or neurodegenerative diseases where splicing errors lead to aberrant protein products. PRP16's interactions with proteins like U2AF may provide additional insights into how its malfunction contributes to disease progression and pathology in such disorders.