Abcam, ab72001, Anti-CENH3 antibody

Size: 200µL
Rabbit Polyclonal CENH3 antibody. Suitable for ICC, WB and reacts with Arabidopsis thaliana samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Arabidopsis thaliana CENH3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Arabidopsis thaliana,
Applications:ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Arabidopsis thaliana CENH3. The exact immunogen used to generate this antibody is proprietary information.Q8RVQ9

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CENH3 also known as Centromere Protein A is a histone H3 variant that plays an essential role in the packaging of centromeric DNA. This protein has a mass of approximately 17 kDa and is uniquely expressed in the centromeric chromatin of eukaryotic cells. CENH3 differs from the canonical H3 histone by having a distinct N-terminal tail which is important for its specific incorporation into centromeres. This specific localization is necessary for the recognition and binding of other centromeric proteins facilitating the assembly of the kinetochore complex.
Biological function summary
CENH3 integrates into nucleosomes at the centromeres forming a specialized chromatin structure critical for chromosome segregation. It contributes to the foundation of the centromere by assembling into a larger protein complex known as the kinetochore. This complex mediates the attachment of spindle microtubules during mitosis ensuring accurate distribution of chromosomes to daughter cells. CENH3's presence at the centromeres helps in recruiting other centromeric proteins such as CENP-C and CENP-N highlighting its central role in stabilizing the centromeric region.
Pathways
CENH3 participates in the cell cycle regulation specifically during mitosis. It acts within the mitotic spindle assembly checkpoint pathway ensuring proper kinetochore function and tension sensing. The mitotic checkpoint kinase MPS1 phosphorylates proteins at the kinetochore and stable CENH3 positioning is necessary for correct attachment. The proper function of CENH3 influences pathways connected to Aurora B kinase activity which affects the correction of misattached microtubules ensuring accurate and efficient mitotic progression.
CENH3 abnormalities can contribute to conditions connected to chromosome missegregation and aneuploidy. Misregulation of CENH3 expression or function links to developmental disorders like cancer and congenital disorders. Dysregulated CENH3 may impact proteins such as SGO1 an essential kinetochore protein that directly affects chromosome cohesion and separation. Abnormal CENH3 expression can result in improper kinetochore function leading to an increased risk of chromosomal instability and tumorigenesis.