Abcam, ab72196, Anti-AKAP 95 antibody

Size: 100µL
Rabbit Polyclonal AKAP 95 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human AKAP8 aa 600 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AKAP8 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O43823

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AKAP95 also known as A-kinase anchor protein 95 is a scaffolding protein with a mass of approximately 85-100 kDa. This protein facilitates the localization of protein kinase A (PKA) to specific subcellular compartments optimizing its function. AKAP95 is expressed in the nucleus of various cell types where it participates in organizing and assembling multi-protein complexes. It plays a mechanical role in linking PKA to specific substrates and other signaling molecules which influences cellular processes.
Biological function summary
AKAP95 integrates into protein complexes that regulate key aspects of cell cycle progression and transcription. It acts in chromatin remodeling which influences gene expression. AKAP95 interacts with several regulatory proteins such as cyclin-dependent kinases to affect cell division. Its role extends to ensuring efficient mitotic entry and chromosome segregation. Through these interactions AKAP95 serves as a central node in the orchestration of nuclear events critical for cell proliferation.
Pathways
AKAP95 operates within signaling networks like the cAMP/PKA pathway and the cell cycle regulation pathway. Through these pathways it connects intimately with proteins such as PKA and cyclin-dependent kinases. AKAP95 binds to these proteins facilitating the orchestration of phosphorylation events that drive cellular responses to external and internal stimuli. These pathways highlight AKAP95’s connectivity in transducing signals necessary for maintaining cellular homeostasis and proliferation control.
AKAP95 shows connections to cancer and cardiac disease. Alterations in its expression and function associate with oncogenic processes involving abnormal cell proliferation commonly seen in cancers. Interactions with proteins like cyclin-dependent kinases highlight its role in tumorigenesis. Additionally dysregulated cAMP/PKA signaling linked to AKAP95 contributes to cardiac dysfunction. Therefore studying AKAP95 in these contexts provides insight into its potential as a biomarker or therapeutic target in these pathological states.