Abcam, ab72264, Anti-CDT2/RAMP antibody

Size: 100µL
Rabbit Polyclonal CDT2/RAMP antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human DTL aa 650 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DTL aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9NZJ0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CDT2 also known as RAMP (Rbx1-Associated Cullin 4-DCAF12-Dependent E3 Ligase) is part of the CRL4 complex and serves as a substrate recognition component for E3 ubiquitin ligases. The molecular weight of the CDT2 protein is approximately 85 kDa. This protein shows expression in various tissues with higher levels in actively dividing cells like those found in the testes and thymus.
Biological function summary
CDT2 regulates cell cycle progression by targeting specific cell cycle regulators for ubiquitin-mediated degradation. It functions within the CRL4 complex where it helps to ensure the timely turnover of replication licensing factors such as Cdt1 and other proteins involved in cell cycle checkpoints. This regulation is important for maintaining genomic stability by preventing inappropriate replication origin firing.
Pathways
CDT2 plays a significant role in DNA replication and cell cycle control pathways. Within these pathways the protein coordinates with Cdt1 to prevent unscheduled DNA replication. CDT2's involvement in these processes links it closely to proteins such as CUL4 which forms part of the CRL4 complex and to the ubiquitin-proteasome system a critical pathway for protein homeostasis.
CDT2 has implications in cancer and genomic instability. Aberrations in CDT2 expression or function can lead to dysregulation of cell proliferation contributing to oncogenesis. Connections between CDT2 and cancer are emphasized by its interaction with substrates like Cdt1 where misregulation may drive the development of tumors. CDT2 is also connected to p21 through its influence on cell cycle checkpoints highlighting an additional role in tumor suppression pathways.