Abcam, ab72385, Anti-GPR177/WLS antibody

Size: 100µL
Chicken Polyclonal GPR177/WLS antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.
Key facts
Host species:Chicken,
Clonality:Polyclonal,
Isotype:IgY,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein L, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GPR177 also known as Wntless (WLS) is a protein that facilitates the transport of Wnt proteins. Wnt proteins are signaling molecules vital for various developmental processes. GPR177/WLS with an approximate mass of 50 kDa is expressed in multiple tissues particularly during early developmental stages. Its role involves the binding and shuttling of Wnt proteins from the Golgi apparatus to the cell surface making it essential for Wnt protein secretion and function.
Biological function summary
GPR177 plays a significant role in the Wnt signaling pathway. This pathway is a fundamental regulator of cellular processes such as proliferation and differentiation. GPR177 is necessary for the proper functioning of this pathway as it forms part of a larger protein complex responsible for Wnt ligand secretion. This complex includes other key proteins like the Evi and Wnt family proteins ensuring the efficient delivery and extracellular presentation of Wnt molecules.
Pathways
The involvement of GPR177 in the Wnt signaling highly influences developmental processes and cellular homeostasis given its pivotal connections to the canonical Wnt/β-catenin pathway and the non-canonical Wnt pathway. GPR177 collaborates specifically with proteins like β-catenin in the canonical pathway where it impacts gene transcription upon Wnt signaling activation. It also associates with signaling components of non-canonical pathways affecting planar cell polarity and calcium signaling which are essential for organismal development and maintenance.
Dysregulation in GPR177 function can contribute to abnormal Wnt signaling linking it to disorders such as cancer and osteoporosis. Abnormal expression or mutation of GPR177 affects the balance of Wnt signaling thereby promoting oncogenic processes in certain cancers by altering β-catenin levels. Moreover impaired Wnt signaling due to GPR177 dysfunction affects bone metabolism connecting it to osteoporosis. Alterations in Wnt pathway components including Frizzled receptors further influence these disease pathways demonstrating the broad impact of GPR177 misregulation.