Abcam, ab72420, Anti-ARFGEF2/BIG2 antibody

Size: 100µL
Rabbit Polyclonal ARFGEF2/BIG2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARFGEF2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARFGEF2. The exact immunogen used to generate this antibody is proprietary information.Q9Y6D5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab72420 was affinity purified using an epitope specific to ARFGEF2/BIG2 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARFGEF2 also known as BIG2 is a guanine nucleotide exchange factor (GEF) that regulates the activation of ADP-ribosylation factors (ARFs). This protein helps in the exchange of GDP to GTP on ARFs which is essential for the function of various cellular processes involving membrane trafficking. ARFGEF2 has a molecular mass of approximately 206 kDa. It is predominantly expressed in the brain particularly in the neurons but also shows expression in other tissues including heart and kidney.
Biological function summary
ARFGEF2/BIG2 is important in maintaining proper Golgi apparatus function and vesicle trafficking. It forms part of various complexes related to vesicle formation and transport within the neuron and other cells. Through its activity ARFGEF2 influences the organization of the Golgi and modulates protein sorting and export. Consequently this protein plays an important role in neural development and cell signaling.
Pathways
ARFGEF2/BIG2 participates actively in the secretory pathway and is a significant player in the trafficking of vesicles from the Golgi apparatus. The protein works closely with ARF proteins facilitating vesicle budding and cargo sorting. Additionally ARFGEF2 interacts with coat protein complex I (COPI) highlighting its role in retrograde transport from the Golgi to the endoplasmic reticulum. It also functions in concert with regulators of membrane curvature to enable proper vesicle formation.
ARFGEF2/BIG2 is linked to the condition known as periventricular heterotopia with microcephaly (PHM) a disorder affecting brain development. Mutations in this protein lead to problems in vesicular trafficking and neuronal migration. Furthermore the improper function of ARFGEF2 can impact the RhoA signaling pathway connected to schizophrenia where RhoA proteins are important linked elements. Defective or altered ARFGEF2 may result in compromised neural structures and predispose individuals to such neurological conditions.