Abcam, ab72560, SureLight® APC Anti-V5 tag antibody

Size: 100µg
Rabbit Polyclonal V5 tag antibody - conjugated to SureLight® APC. Suitable for Flow Cyt, sELISA and reacts with Tag samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Conjugation:SureLight® APC,
Excitation/Emission:Ex: 652nm, Em: 657nm,
Carrier free:No,
Applications:Flow Cyt, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Excitation maximum: 650 nm Emission maximum: 660 nm

Properties and Storage Information:
Form-Liquid, Purification technique-Size-exclusion chromatography, Purification notes-Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used., Storage buffer-pH: 7.4Preservative: 0.013% Sodium azideConstituents: 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
Biological function summary
Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.
Pathways
The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.
Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.