Abcam, ab74065, Anti-MASH1/Achaete-scute homolog 1 antibody

Size: 100µg
Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) is a rabbit polyclonal antibody detecting MASH1/Achaete-scute homolog 1 in Western Blot, ICC/IF . Suitable for Human, Mouse, Rat, Zebrafish . - Over 40 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Zebrafish,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Ab74065 has been batch tested in WB and IF only. Some customers have successfully used this product in IHC. Please contact Abcam Scientific Support for more information.

Product details:
What is this antibody validated in?
Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat, Zebrafish samples.
What is the molecular weight of MASH1/Achaete-scute homolog 1?
Anti-MASH1/Achaete-scute homolog 1 (ab74065) specifically detects a band for MASH1/Achaete-scute homolog 1 (UniProt: P50553) at a molecular weight of 25kDa.
Trusted by the scientific community
Anti-MASH1/Achaete-scute homolog 1 (ab74065) was first used in a scientific publication in 2009 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-MASH1/Achaete-scute homolog 1 (ab74065) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.
Biological function summary
This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.
Pathways
MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.
MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.