Abcam, ab74473, Anti-Borealin/CDCA8 antibody

Size: 100µg
Rabbit Polyclonal Borealin/CDCA8 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Borealin also known as CDCA8 is a component of the chromosomal passenger complex (CPC) with a mass of approximately 22 kDa. This protein facilitates proper chromosome segregation and cytokinesis during cell division. It is mainly expressed in proliferating cells especially in tissues undergoing frequent cell cycles like testis and tumor tissues. Borealin localizes to the centromeres and the spindle midzone during mitosis highlighting its mechanical involvement in maintaining chromosome alignment and proper cell division.
Biological function summary
Borealin serves as an important element in ensuring accurate mitosis. It functions as an integral part of the chromosomal passenger complex which also includes Aurora B kinase Survivin and INCENP. This complex regulates chromosome-microtubule interactions and cytokinesis. Borealin interacts with other CPC components to ensure stability and proper localization of the complex facilitating important mitotic functions. This highlights Borealin's biological role in maintaining genomic stability.
Pathways
Borealin integrates into the cell cycle and mitotic pathways. These pathways include the regulation of Aurora B kinase and its associated proteins such as Survivin and INCENP playing a critical role in chromosome condensation spindle assembly and cytokinesis. In the mitotic pathway Borealin's interaction with the CPC allows for precise coordination of mitotic events providing a framework for successful cell division and its associated processes.
Abnormalities in Borealin function are linked to cancer and chromosomal instability syndromes. Disrupted Borealin expression or function can lead to abnormal mitosis and contribute to tumorigenesis. Research suggests that Borealin alterations may relate to other proteins involved in cancer including Aurora B kinase influencing cancer progression through enhanced genomic instability or proliferation rates. Understanding Borealin's contribution to these diseases may offer new avenues for therapeutic exploration in targeting cancer cell division.