Abcam, ab7538, Native Human Collagen VI protein

Size: 500µg
Native Human Collagen VI protein is a Human Full Length protein with >95% and suitable for ELISA, IP, IHC, SDS-PAGE and western blot. - Save time and ensure accurate results - use our Collagen VI (COL6A1-COL6A3) protein as a control -Does not cross react with other collagens
Key facts
Purity:>95%,
Expression system:Native,
Tags:Tag free,
Applications:IP, IHC-P, SDS-PAGE, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P12109,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:Preservative: 0.01% Sodium azideConstituents: 3% Acetic acid

Product details:
The Native Human Collagen VI protein ab7538 has been prepared from human placenta and is chromatographically and immunologically pure.
This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with anti-Collagen I, II, III, IV or V is negligible (typically less than 1% cross reactivity was detected by ELISA).
Ensure the validity of your result using our Collagen VI protein ab7538 as a control in western blot and SDS-PAGE. Collagen type VI is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
The recommended buffer for SDS-PAGE to use with ab7538 is 2X SDS buffer with 8 M Urea. The low pH of the buffer maintains the solubility of the collagen proteins. Also, low-density gels such as 3-8% Tris Acetate should be used. Collagen proteins can be reduced with DTT.
Check out our protein gel staining guide for SDS-PAGE
Check out of western blot protocol for more information
Check out our ELISA protocol for more information

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Diluted stock solutions should be used immediately and not be frozen, Store undiluted

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen VI also known as Collagen 6 is a type of collagen that plays an important role in the extracellular matrix. It consists of three different chains: α1(VI) α2(VI) and α3(VI) with a combined mass of approximately 140 kDa. This collagen type strongly associates with several tissues notably in skeletal muscle skin and the walls of blood vessels. Scientists often study collagen VI with specific collagen antibodies to understand its unique structural properties and its end which binds to other matrix components.
Biological function summary
Collagen VI contributes to structural integrity and cellular scaffolding within tissues. This protein is integral to the formation of microfibrillar networks part of a larger complex that connects the extracellular matrix to cells and other matrix components. Its protective role helps cells resist mechanical stress and the network provided by collagen VI powder stabilizes the tissue architecture. Additionally low endotoxin collagen is often preferable in research to minimize inflammatory responses during experimentation providing more accurate results.
Pathways
Collagen VI participates in extracellular matrix organization and mechanotransduction pathways. It functions alongside other proteins like collagen I and IV. In particular collagen VI interacts with cell surface receptors which influences cellular communication and signaling pathways. This interaction plays a role in transmitting mechanical signals that affect cellular behavior and function proving important for tissue maintenance and repair processes.
Mutations or malfunctions involving collagen VI can lead to significant clinical outcomes. It is prominently linked with disorders such as Ullrich congenital muscular dystrophy and Bethlem myopathy both affecting muscle and connective tissue. These conditions often involve disruptions in the assembly or function of the collagen VI network. Additionally related proteins like collagen I and integrin proteins can influence the progression and severity of these disorders illustrating the interconnected nature of these extracellular elements.