Abcam, ab7549, Anti-NFkB p105 / p50 antibody

Size: 100µL
Rabbit Polyclonal NFkB p105 / p50 antibody. Suitable for EMSA, ELISA, WB, IHC-P, GSA and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human NFKB1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, GSA, IHC-P, EMSA, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NFKB1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P19838,
Specificity:Recognition is at the N-terminus. This antibody will "supershift " NFkB complexes containing the human p50 subunit. Control peptide ab7550 will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1).

Product details:
NFkB is formed as a homo- or hetero-dimer. Subunits include p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Purification notes-This product was prepared from monospecific antiserum by delipidation and defibrination., Storage buffer-Preservative: 0.01% Sodium azideConstituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle