Abcam, ab75508, Anti-MPRA antibody

Size: 100µg
Rabbit Polyclonal MPRA antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Mechanical Properties of MPRA are noteworthy. MPRA also known as Massively Parallel Reporter Assays is not a protein but an experimental technique used to study enhancer activity on a large scale. It does not have a specific mass or expression pattern because it is a method rather than a molecule. Researchers often use it to understand how different combinations of enhancer sequences affect transcriptional activity across multiple genes. MPRA encompasses a large DNA library with many variants which researches transfect into cells to analyze concurrent expression changes.
Biological function summary
MPRA helps to unravel gene regulatory networks starting with regulatory elements. It does not participate in a classic sense of the term as part of a protein complex but plays a role in testing and validating the activity of enhancers and promoters within the genomic context. The assay is important for identifying non-coding elements that modulate gene expression which aids in understanding gene control mechanisms. Scientists often utilize the method to focus on elucidating interactions at cellular levels bridging gaps in comprehensive genomic annotations.
Pathways
This methodology extensively aids in deciphering transcriptional regulatory pathways important for gene expression decisions. Scientists employ MPRA to explore how enhancer elements modulate pathways like cell signaling and metabolic processes by influencing transcription factors such as CREB and NF-kB. It connects with relevant mechanisms by highlighting upstream enhancer roles in gene expression pathways revealing interaction with proteins that guide developmental and homeostatic pathways.
MPRA's analytical strength aids in linking genetic elements with specific conditions. Researchers have used it to investigate the implications of enhancer mutations in diseases like cancer and neurological disorders. By recognizing enhancer alterations associated with these diseases MPRA works in association with proteins such as p53 and GATA3 clarifying their roles in pathogenesis. The method empowers studies aiming to reveal malfunctioning genetic circuits that contribute to the onset and progression of various diseases.