Abcam, ab75745, Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody

Size: 100µL
Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) is a rabbit polyclonal antibody detecting GSK3 alpha in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 160 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.Synthetic Peptide within Human Glycogen synthase kinase-3 alpha phospho Y279. Database linkP49840,
Specificity:ab75745 detects endogenous levels of GSK3 beta only when phosphorylated at tyrosine 216 and GSK3 alpha when phosphorylated at tyrosine 276. This antibody can also cross-react with GSK3 alpha subunit due to the consistent antigen-sequence of "GEPNVSYICSRYY".

Product details:
What is this antibody validated in?
Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of GSK3 alpha?
Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) (ab75745) specifically detects a band for GSK3 alpha (UniProt: P49840) at a molecular weight of 47kDa.
Trusted by the scientific community
Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) (ab75745) was first used in a scientific publication in 2009 and has been cited over 160 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab75745 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycogen synthase kinase 3 beta (GSK3Β) and glycogen synthase kinase 3 alpha (GSK3α) are serine/threonine protein kinases involved in various cellular processes. GSK3Β weighs approximately 47 kDa while GSK3α is around 51 kDa. These proteins are expressed in various tissues including brain heart and liver. GSK3 isoforms act as key regulators in signaling pathways by phosphorylating target proteins affecting their function and stability. Alternative names for GSK3 include GSK-3Β and GSK-3α respectively.
Biological function summary
The kinases play essential roles in cellular functions like glycogen metabolism cell cycle regulation and apoptosis. GSK3 proteins often function as part of larger enzyme complexes influencing cellular responses to external signals. They bind to numerous substrates impacting processes such as glucose homeostasis and neuron function. Dramatic changes in GSK3 activity can disrupt these biological processes highlighting their importance.
Pathways
These kinases are integral components of the Wnt/Β-catenin and insulin signaling pathways. In the Wnt pathway GSK3 is involved in Β-catenin phosphorylation affecting its degradation and transcriptional activity. In the insulin pathway GSK3 influences glycogen synthesis by phosphorylating and regulating glycogen synthase. Both pathways highlight how GSK3 interacts with other proteins such as adenomatous polyposis coli (APC) and axin to coordinate cellular activities.
GSK3 has links to conditions like Alzheimer's disease and diabetes mellitus. Dysregulation of GSK3 activity is observed in Alzheimer's disease where abnormal GSK3 signaling contributes to tau hyperphosphorylation and neurofibrillary tangle formation. In diabetes altered GSK3 function affects insulin signaling pathways influencing glucose metabolism. GSK3's involvement in these diseases often connects it to proteins like tau in Alzheimer's and insulin receptor substrate 1 (IRS1) in diabetes.