Abcam, ab75941, Anti-Cytochrome b245 Light Chain/p22-phox antibody

Size: 100µg
Rabbit Polyclonal Cytochrome b245 Light Chain/p22-phox antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 32 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Replenishment batches of ab75941 are tested in WB. Previous batches were additionally validated in IHC-P. This application is still expected to work and is covered by our Abpromise guarantee. Please note, previous lots of this product were also guaranteed in ICC/IF. We are having trouble reproducing new lots suitable for ICC/IF. Therefore this application has been removed and it no longer guaranteed.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Cytochrome b245 Light Chain also known as p22-phox or CYBA plays a significant role in the NADPH oxidase complex. This protein has a molecular mass of approximately 22 kDa and is widely expressed in various cell types particularly in those associated with the immune system such as neutrophils. p22-phox forms part of the membrane-bound flavocytochrome b558 along with gp91-phox which is important for the generation of reactive oxygen species.
Biological function summary
The p22-phox serves as a core component of the NADPH oxidase complex which is integral to cellular responses to infection and stress. It operates in conjunction with other cytosolic subunits to produce reactive oxygen species that help in microbial destruction and signal transduction. The presence of p22-phox is essential for proper enzyme assembly and function as its interaction with gp91-phox establishes the electron transport chain required for oxidase activity.
Pathways
P22-phox contributes to the oxidative burst pathway a critical mechanism in innate immunity. In this pathway its interactions with proteins like Rac a small GTPase facilitate the activation and membrane association of the oxidase complex. Additionally p22-phox has involvement in inflammatory pathways by aiding in the release of signaling molecules that trigger inflammatory responses. This protein cooperates with components of the MAPK signaling pathway acting as a mediator in signal transduction events that dictate cellular responses to external stimuli.
P22-phox links closely with chronic granulomatous disease (CGD) a disorder characterized by a malfunctioning NADPH oxidase complex. Mutations in the CYBA gene can lead to defects in reactive oxygen species generation compromising the immune system's ability to combat infections. Furthermore evidence suggests a connection between p22-phox and cardiovascular diseases including hypertension where abnormalities in oxidase activity influence endothelial function. Proteins such as gp91-phox also share these disease associations highlighting the cooperative interaction of these components in disease pathology.