Abcam, ab75985, Anti-CD21 antibody [EP3093]

Size: 100µL / 1mL
Anti-CD21 antibody [EP3093] (ab75985) is a rabbit monoclonal antibody detecting CD21 in Western Blot, IP, IHC-P, ICC/IF . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP3093,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-CD21 antibody [EP3093] (ab75985) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of CD21?
Anti-CD21 [EP3093] (ab75985) specifically detects a band for CD21 (UniProt: P20023) at a molecular weight of 113kDa.
Trusted by the scientific community
Anti-CD21 [EP3093] (ab75985) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-CD21 [EP3093] (ab75985) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EP3093] also available for your convenience: ab75985, Carrier free -
ab193554
, HRP -
ab202354
, Alexa Fluor® 488 -
ab202692
, Alexa Fluor® 647 -
ab202693
, Carrier free -
ab271855
, Alexa Fluor® 594 -
ab281930
, Alexa Fluor® 555 -
ab282007
, Biotin -
ab322303
, Alexa Fluor® 555 - ab322950
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.
Biological function summary
Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.
Pathways
CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.
CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.