Abcam, ab75991, Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]

Size: 20µL / 100µL / 1mL
Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) is a rabbit monoclonal antibody detecting PKA alpha/beta/gamma (catalytic subunit) in Western Blot, IHC-P, Dot Blot . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2606Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:Dot, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognizes the T197 phospho-site, which is the same site as the T198 on Uniprot P22612.Detects PKA alpha/beta/gamma (catalytic subunit) phosphorylated on threonine 197. Moreover, the antibody will cross-react with PRKX/Y (pT203).

Product details:
What is this antibody validated in?
Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse, Rat samples.
What is the molecular weight of PKA alpha/beta/gamma (catalytic subunit)?
Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) [EP2606Y] (ab75991) specifically detects a band for PKA alpha/beta/gamma (catalytic subunit) (UniProt: P17612) at a molecular weight of 40kDa.
Trusted by the scientific community
Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) [EP2606Y] (ab75991) was first used in a scientific publication in 2009 and has been cited over 50 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EP2606Y] also available for your convenience: ab75991, Carrier free -
ab227848
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein kinase A (PKA) alpha/beta/gamma also known as the PKA catalytic subunit plays a significant role in the regulation of cellular functions through phosphorylation. It has alternate names including phospho PKA and PKA C alpha with an approximate molecular weight of 40 kDa. The PKA catalytic subunit is expressed in various tissues throughout the body. This distribution highlights its broad physiological impact given PKA's involvement in numerous cellular processes.
Biological function summary
PKA alpha/beta/gamma acts as an important regulatory molecule in signal transduction pathways. It functions by phosphorylating serine and threonine residues on target proteins which alters the activity protein interactions or localization of these substrates. PKA functions as part of the cAMP-dependent PKA holoenzyme complex which includes two regulatory (R) and two catalytic (C) subunits. Activation requires the binding of cAMP to the R subunits causing dissociation and releasing the active C subunits to exert their effects.
Pathways
PKA alpha/beta/gamma integrates into the cAMP signaling pathway which modulates numerous physiological processes such as metabolism gene expression and cell growth. In the cAMP pathway PKA interacts closely with proteins like adenylyl cyclase and phosphodiesterases which respectively increase and decrease cAMP levels affecting PKA activation state and subsequent cellular responses. PKA also plays roles within the MAPK/ERK pathway where it intersects with other kinases to influence cell cycle progression and differentiation.
PKA alpha/beta/gamma has connections to cancer and cardiovascular diseases. Mutations or dysregulation of PKA activity can lead to abnormal cell proliferation and survival contributing to tumorigenesis. The catalytic subunit is also linked to cardiovascular disorders through its impact on heart rate and contractility. Interaction with proteins such as AKAPs (A-kinase anchoring proteins) can localize PKA activity affecting local signaling cascades that influence disease pathology.