Abcam, ab76003, Anti-MMP9 antibody [EP1254]

Size: 100µL / 1mL
Anti-MMP9 antibody [EP1254] (ab76003) is a rabbit monoclonal antibody detecting MMP9 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Rat, . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 550 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1254,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:IHC-P, ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Based on our preliminary data, ab76003 detects no or weak band of interest in the untreated cell lines at the dilution of 1/200. Treatment increasing the expression of MMP-9 is recommended when using this antibody.This antibody works better in 1% SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/orhere (downloadable copy).

Product details:
Product Specifications
Anti-MMP9 antibody [EP1254] (ab76003) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human, rat, samples.
Anti-MMP9 antibody [EP1254] (ab76003) specifically detects MMP9 (UniProt ID: P14780; Molecular weight: 1kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-MMP9 antibody [EP1254] (ab76003) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-MMP9 antibody [EP1254] (ab76003) has been cited over 551 times in peer reviewed journals and is trusted by the scientific community.
Anti-MMP9 antibody [EP1254] (ab76003) has 12 independent reviews from customers.
Related Products
Antibody clone EP1254 is also available pre-conjugated to a variety of labels for your convenience - PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 750 (
ab305635
ab305636
ab305637
ab308802
ab309755
ab310122
ab310533
ab312063
ab312540
ab321706
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
Biological function summary
Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.
Pathways
MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.
Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.