Abcam, ab76113, Anti-Prostate Specific Antigen antibody [EP1588Y]

Size: 20µL / 100µL / 1mL
Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) is a rabbit monoclonal antibody detecting Prostate Specific Antigen in Western Blot, IHC-P, mIHC . Suitable for Human . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1588Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The antibody provided good results in western blot when tested in human prostate lysate. Unfortunately, when 22RV1 cell lysate was used no band was detected and inconsistent results were obtained with LNCaP cell lysate (where different lots of cell lysate were used for the test). Please contact our Scientific Support if you have any feedback and/or questions.

Product details:
What is this antibody validated in?
Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Multiplex IHC (mIHC) in Human samples.
What is the molecular weight of Prostate Specific Antigen?
Anti-Prostate Specific Antigen [EP1588Y] (ab76113) specifically detects a band for Prostate Specific Antigen (UniProt: P07288) at a molecular weight of 29kDa.
Trusted by the scientific community
Anti-Prostate Specific Antigen [EP1588Y] (ab76113) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EP1588Y] also available for your convenience: ab76113, Carrier free -
ab215983
, Carrier free -
ab271858
, APC -
ab319198
, PE -
ab319346
, Alexa Fluor® 488 -
ab319480
, Alexa Fluor® 647 -
ab319593
, Alexa Fluor® 594 -
ab319697
, Alexa Fluor® 555 -
ab319839
, Alexa Fluor® 750 -
ab321717
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.
Biological function summary
PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.
Pathways
PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.
PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.