Abcam, ab76128, Anti-Tau antibody [EP2456Y]

Size: 20µL / 100µL / 1mL
Anti-Tau antibody [EP2456Y] (ab76128) is a rabbit monoclonal antibody detecting Tau in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2456Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody clone has been shown to react with Tau 4R and 3R isoforms (doi.org/10.1101/2023.04.13.536711).Our testing suggests that this antibody clone does not cross-reacts with MAP2 or MAP4

Product details:
Product Specifications
Anti-Tau antibody [EP2456Y] (ab76128) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, WB in human, mouse, rat samples.
Anti-Tau antibody [EP2456Y] (ab76128) specifically detects Tau (UniProt ID: P10636; Molecular weight: 79kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Tau antibody [EP2456Y] (ab76128) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Tau antibody [EP2456Y] (ab76128) has been cited over 22 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Antibody clone EP2456Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, PE, Alexa Fluor® 555 (
ab206060
ab210985
ab215290
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.