Abcam, ab76148, Anti-MSI2 antibody [EP1305Y]

Size: 20µL / 100µL / 1mL
Anti-MSI2 antibody [EP1305Y] (ab76148) is a rabbit monoclonal antibody detecting MSI2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1305Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, Flow Cyt (Intra), ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:Based on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2.

Product details:
What is this antibody validated in?
Anti-MSI2 antibody [EP1305Y] (ab76148) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of MSI2?
Anti-MSI2 [EP1305Y] (ab76148) specifically detects a band for MSI2 (UniProt: Q96DH6) at a molecular weight of 35kDa.
Trusted by the scientific community
Anti-MSI2 [EP1305Y] (ab76148) was first used in a scientific publication in 2009 and has been cited over 40 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-MSI2 antibody [EP1305Y] (ab76148) has been confirmed by Western blot testing in MSI2 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EP1305Y] also available for your convenience: ab76148, Alexa Fluor® 488 -
ab199067
, Alexa Fluor® 647 -
ab199251
, HRP -
ab199252
, Carrier free -
ab221789
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Musashi-2 (MSI2) is a member of the Musashi RNA-binding protein family also labeled as MSI-2. The protein is approximately 38 kDa in size. It expresses primarily in stem cells and various tumor types including hematopoietic cells and neural tissue. MSI2 plays a role in maintaining stem cell self-renewal and differentiation by regulating mRNA translation through binding to specific target RNAs.
Biological function summary
MSI2 contributes to the regulation of gene expression with a significant impact on cell fate decisions. It acts as part of a post-transcriptional regulatory complex that controls the stability and translation of specific mRNA targets. MSI2 influences pathways involved in stem cell maintenance and differentiation linking it to cellular processes like proliferation and apoptosis. Its activity is important for normal development and the maintenance of undifferentiated cell states.
Pathways
MSI2 has important roles in the Notch and Wnt signaling pathways. These pathways are vital for stem cell maintenance and tissue regeneration. MSI2 regulates components of these pathways interacting with proteins such as beta-catenin in Wnt signaling. This regulation enhances its involvement in controlling cell proliferation and differentiation contributing to signaling processes that determine cellular behavior.
MSI2 is linked to acute myeloid leukemia (AML) and certain types of solid tumors. Overexpression of MSI2 often correlates with poor prognosis in AML where it impacts cancer progression by modulating genes associated with cell growth. Additionally its interaction with oncogenic proteins like BCR-ABL suggests its involvement in cancerogenesis through disease-linked pathways. Research indicates MSI2 as a potential target for therapeutic intervention in such malignancies.