Abcam, ab76236, Anti-ERCC1 antibody [EP2143Y]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ERCC1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2143Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab76236 might also detect a heterodimer composed of ERCC1 and XPF/ERCC4 at 100-150 kDa.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ERCC1 also known as Excision Repair Cross-Complementation Group 1 is an essential protein in DNA repair mechanisms. Weighing approximately 36 kDa ERCC1 is expressed in a variety of tissues throughout an organism. It carries out its function by forming a heterodimer with XPF which is a structure-specific endonuclease playing a vital role in the nucleotide excision repair (NER) pathway that helps maintain genomic stability. The presence of an antibody like 8F1 can be helpful for detecting the ERCC1 protein expression in biological samples through techniques like ELISA.
Biological function summary
ERCC1 plays a significant role in the DNA damage repair process where it interacts with its partner protein XPF to excise damaged DNA. The ERCC1-XPF complex recognizes and cuts both single- and double-stranded DNA therefore enabling the removal of DNA lesions caused by UV radiation or chemical mutagens. This action prevents mutations by ensuring accurate DNA replication and transcription.
Pathways
The ERCC1 protein is primarily involved in the nucleotide excision repair (NER) and interstrand cross-link repair pathways. Within these pathways ERCC1 provides repair capacity to the cell maintaining the functionality of the DNA by partnering with proteins such as XPF and participating in cross-link repair mechanisms. By coordinating with proteins like XPA and RPA ERCC1 assists in identifying and binding the damaged DNA facilitating the recruitment of other repair proteins.
The ERCC1 protein is linked to conditions such as Xeroderma Pigmentosum and Fanconi Anemia largely due to its critical role in DNA repair. Malfunctions or deficiencies in ERCC1 or its partner proteins can lead to compromised DNA repair resulting in susceptibility to cancer and other genomic instability disorders. ERCC1's relationship with proteins that partake in cell cycle regulation further emphasizes its impact on disease progression linking it with potential therapeutic targets for addressing these genetic disorders.