Abcam, ab76252, Anti-YAP1 (phospho S127) antibody [EP1675Y]

Size: 20µL / 100µL / 1mL
Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) is a rabbit monoclonal antibody detecting YAP1 in Western Blot, IHC-P, Dot Blot . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 100 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1675Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Dot, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.

Product details:
What is this antibody validated in?
Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse, Rat samples.
What is the molecular weight of YAP1?
Anti-YAP1 (phospho S127) [EP1675Y] (ab76252) specifically detects a band for YAP1 (UniProt: P46937) at a molecular weight of 65 - 75kDa.
Trusted by the scientific community
Anti-YAP1 (phospho S127) [EP1675Y] (ab76252) was first used in a scientific publication in 2009 and has been cited over 100 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EP1675Y] also available for your convenience: ab76252, Carrier free -
ab172374
, Alexa Fluor® 488 -
ab310956
, Alexa Fluor® 647 -
ab311073
, Alexa Fluor® 568 -
ab312934
, Alexa Fluor® 555 -
ab313146
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
YAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.
Biological function summary
YAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.
Pathways
YAP1 is importantly involved in the Hippo signaling pathway and Wnt/Β-catenin signaling pathway. In the Hippo pathway kinases LATS1/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt/Β-catenin pathway YAP1 interacts with Β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.
YAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-Β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.