Abcam, ab76779, Anti-RAB27B antibody

Size: 50µg
Mouse Polyclonal RAB27B antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAB27B.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human RAB27B.O00194

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB27B also known as Ras-related protein Rab-27B belongs to the Rab family of small GTPases and has a molecular mass of approximately 25 kDa. RAB27B is involved in intracellular vesicle trafficking processes. It is expressed in various tissues with notable expression in platelets and secretory cells. The protein cycles between GDP-bound inactive and GTP-bound active states regulates cargo delivery and maintains distinct cellular compartments.
Biological function summary
The functional role of RAB27B includes mediating the transport and exocytosis of secretory vesicles. This protein collaborates with effector molecules such as MLPH (melanophilin) as part of a multiprotein complex that facilitates vesicle membrane docking and fusion. RAB27B's activity ensures proper cellular secretion processes impacting hormone release pigment distribution and other essential secretory pathways.
Pathways
RAB27B participates in the exosome secretion and regulated secretory pathways. It closely interacts with other proteins including RAB27A which shares similar trafficking functions. RAB27B plays a role in specialized secretory pathways affecting the maturation and docking of vesicles in cells like melanocytes and cytotoxic T lymphocytes which are essential for efficient immune responses and pigmentation respectively.
RAB27B is relevant in conditions such as cancer and Griscelli syndrome. Its dysregulation can contribute to tumor progression and metastasis due to altered vesicle trafficking and secretion dynamics. In Griscelli syndrome type 2 mutations in the related protein RAB27A although not directly in RAB27B impact melanosome transport leading to pigmentary issues and immune dysfunction. Understanding RAB27B's involvement offers insights into potential therapeutic strategies targeting these pathways.