Abcam, ab76841, Anti-gp78 antibody

Size: 100µL
Rabbit Polyclonal gp78 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human AMFR.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-Fr, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AMFR. The exact immunogen used to generate this antibody is proprietary information.Q9UKV5,
Specificity:ab76841 reacts specifically with the 73 kda (isoform 2) protein.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gp78 also known as AMFR (Autocrine Motility Factor Receptor) functions as an E3 ubiquitin ligase within the endoplasmic reticulum (ER). This protein exhibits a mass of around 78 kDa. You can find gp78 expressed in several tissues but with strong presence in the liver. It is integral to the ER-associated degradation (ERAD) pathway where it tags misfolded proteins with ubiquitin for subsequent degradation by the proteasome maintaining protein quality control within cells.
Biological function summary
Gp78 acts as a regulator of protein degradation by forming a complex with other proteins like Ubc7 and Derlin-1. It ensures the balance between protein synthesis and degradation. Gp78 aids in dislocating misfolded proteins from the ER lumen into the cytosol facilitating their destruction. Through its E3 ligase activity gp78 helps control cellular protein levels reducing the stress on cells when protein folding is imperfect.
Pathways
Gp78 plays a role in the ubiquitin-proteasome pathway and the ERAD pathway. These pathways manage protein turnover and ER stress response. In these processes gp78 interacts with proteins such as VCP/p97 which assists in extracting the ubiquitinated proteins from the ER membrane. This cooperation highlights gp78’s involvement in maintaining cellular homeostasis helping cells adapt to changes in the ER environment.
Gp78 shows a connection to cancer and neurodegenerative diseases. Overexpression of gp78 has been linked to metastasis in cancer where it may influence the regulation of cell motility and invasion capabilities. Additionally alterations in the activity of gp78 have implications in neurodegenerative disorders like Parkinson's disease where misfolded protein accumulation is problematic. In these contexts gp78 interacts with proteins such as Parkin an E3 ligase also involved in protein degradation further emphasizing its role in disease-related protein quality control.