Abcam, ab76923, Anti-MED13/GCT antibody

Size: 100µL
Rabbit Polyclonal MED13/GCT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MED13 aa 300-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MED13 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.Q9UHV7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MED13 also known as Mediator complex subunit 13 is a significant protein component of the Mediator complex which is important for transcription regulation by RNA polymerase II. This protein with a mass around 200 kDa is widely expressed in various tissues including the liver heart and kidney. MED13 interacts with multiple transcriptional activators and is integral to the assembly and stability of the Mediator complex influencing gene expression.
Biological function summary
Mediator complex subunit 13 acts as a gatekeeper in gene expression regulation by integrating signals from transcription factors to initiate transcription by RNA polymerase II. MED13 is an essential part of the CDK8 submodule within the Mediator complex impacting its regulatory role in transcription activation and repression. Its function tightly connects to the control of cell growth development and metabolism impacting how genes are expressed in response to cellular signals.
Pathways
MED13 plays an important role in the Wnt/β-catenin and nuclear receptor signaling pathways. These pathways impact the regulation of development cell proliferation and metabolic processes. MED13 coordinates with proteins like CDK8 visiting these pathways to influence transcriptional outcomes. Through these coordinated actions MED13 helps regulate signals that guide cellular behavior and differentiation.
Disruptions in the function of MED13 can contribute to various conditions. Abnormal MED13 activity has been linked to heart diseases specifically cardiac hypertrophy and metabolic disorders like obesity. In the context of these diseases MED13 interacts with other proteins like GATA4 and PPARγ which play roles in the pathological processes underlying these conditions. Understanding MED13's involvement in these disorders offers important insights into potential therapeutic interventions.