Abcam, ab7754, Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker

Size: 100µg
Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754) is a mouse monoclonal antibody detecting Cytokeratin 19 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Over 50 publications - Trusted since 2001
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:A53-B/A2,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing KRT19 protein. The exact immunogen used to generate this antibody is proprietary information.P08727,
Epitope:Rod domain of cytokeratin peptide 19.,
Specificity:Rod domain of cytokeratin peptide 19 (40 kDa) in human tissue.

Product details:
What is this antibody validated in?
Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Cytokeratin 19?
Anti-Cytokeratin 19 [A53-B/A2] - Cytoskeleton Marker (ab7754) specifically detects a band for Cytokeratin 19 (UniProt: P08727) at a molecular weight of 44kDa.
Trusted by the scientific community
Anti-Cytokeratin 19 [A53-B/A2] - Cytoskeleton Marker (ab7754) was first used in a scientific publication in 2001 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-Cytokeratin 19 [A53-B/A2] - Cytoskeleton Marker (ab7754) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754) has been confirmed by Immunocytochemistry testing in Cytokeratin 19 Knockout MCF7 cells.
Other related products
We have a range of other formats of antibody clone [A53-B/A2] also available for your convenience: ab7754, FITC -
ab178543

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytokeratin 19 (CK19) also known as cytokera­tine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.
Biological function summary
CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.
Pathways
CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.
CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.