Abcam, ab776, Anti-HMW Cytokeratin antibody [34bE12]

Size: 500µL
Mouse Monoclonal Cytokeratin 5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:34bE12,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.3 - 7.5Preservative: 0.05% Sodium azideConstituents: Tissue culture supernatant, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HMW Cytokeratin also known as High Molecular Weight Cytokeratin refers to a group of keratin proteins typically around 66-68 kDa that forms part of the cytoskeleton in epithelial cells. These proteins express mainly in stratified squamous epithelia and some simple epithelia providing structural integrity and resistance to mechanical stress. They occur in epithelial tissues like the skin mouth esophagus and bladder playing a role in cellular differentiation and tissue organization.
Biological function summary
High molecular weight cytokeratins contribute significantly to the structural framework of cells. They form intermediate filaments that provide mechanical support and maintain cell shape. As part of this filament complex HMW Cytokeratins interacts with other cytoskeletal structures and adhesion molecules taking an active part in cellular dynamics and stability. These keratins also assist in the protection of epithelial cells from mechanical and non-mechanical stress.
Pathways
HMW Cytokeratins plays an important role in the keratinization pathway and epithelial cell differentiation processes. In the keratinization pathway these proteins interact with other keratins like KRT5 and KRT14 helping in the formation of resilient and tough surfaces on epithelial cells. In epithelial cell differentiation they are important in the regulation of cellular processes that define cell fate and tissue structure engaging with proteins like p63 which regulates gene expression in epithelial stem cells.
High molecular weight cytokeratins associates with several epithelial-derived cancers including squamous cell carcinoma and skin-related disorders such as ichthyosis. Aberrant expression or mutation in these keratin genes can lead to impaired epithelial cell function or transformation. In certain cancers HMW Cytokeratins often coexpress with proteins like vimentin indicating epithelial-mesenchymal transition and aiding in cancer diagnosis and prognosis.